Friday, December 10, 2010
Protein localization using electron and fluorescence nanoscopy
Molecular topography of a cell can be successfully monitored by combining powerful imaging techniques such as electron microscopy and fluorescence nanoscopy ( STED or PALM). Recently, Prof. Erik M Jorgensen and his colleagues described a correlative fluorescence electron microscopy technique to localize protein on specific organelles. Here organelles first are revealed by electron microscopy and proteins are monitored by fluorescence imaging. As a result of image correlation between these two imaging modalities, proteins can be localized with nanometer accuracy. The paper also demonstrates localization of histone proteins on mitochondria.
Here is the paper that was published in Nature Methods: "Protein localization in electron micrographs using fluorescence nanoscopy"